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5c–d). CX-5461-mediated S33 phosphorylation of RPA was impartial with the mobile cycle stage and wasn't limited into the nucleoli in HR-deficient cells. Consequently, the development of ssDNA constructions in CX-5461 treated cells can cause replication fork stalling and ATR activation with HRD exacerbating CX-5461-mediated replication anxiety a